All these facts have led to a great expansion of RNA-seq, becoming the first choice in transcriptomic analysis for many scientists. RNA-seq also offers a high degree of agreement with other techniques considered as the gold standard in transcriptomics such as qRT-PCR, both at absolute and relative gene expression measurement levels 7. Compared with microarrays, RNA-seq enables the study of novel transcripts and offers higher resolution, a better range of detection and lower technical variability 5, 6. In recent years, RNA-seq has emerged as an alternative method to that of classic microarrays for transcriptome analysis 1, 2, 3, 4. This study weighs up the advantages and disadvantages of the tested algorithms and pipelines providing a comprehensive guide to the different methods and procedures applied to the analysis of RNA-seq data, both for the quantification of the raw expression signal and for the differential gene expression. The procedures were validated by qRT-PCR in the same samples. Differential gene expression performance was estimated by testing 17 differential expression methods. Raw gene expression signal was quantified by non-parametric statistics to measure precision and accuracy. In the present study, 192 pipelines using alternative methods were applied to 18 samples from two human cell lines and the performance of the results was evaluated. Consequently, there is no clear consensus about the most appropriate algorithms and pipelines that should be used to analyse RNA-seq data. This has resulted in a substantial increase in the number of options available at each step of the analysis. ![]() ![]() As the analysis of RNA-seq data is complex, it has prompted a large amount of research on algorithms and methods. RNA-seq is currently considered the most powerful, robust and adaptable technique for measuring gene expression and transcription activation at genome-wide level.
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Fowler getting everyone's attention during the meeting."Turn your chicken farm into a gold mine!" And it even continues the joke by being aimed right at her situation with an ad for the pie machine."I'm sick and tired of making minuscule profits!" (Notices a magazine with the headline " Sick and tired of making minuscule profits?").and even goes right back to making it after an off-screen crash momentarily distracts her. She later makes the same face when one of Rocky's flying lessons fails miserably. Ginger's very sarcastic smile when Rocky tells her she shouldn't turn him in because he's cute, right before she squawks to get him caught.Fowler falling over while covered by the sock.For emphasis, they even animated her uvula when she yells "tripe"!.She posted a video of herself recreating some of the lines from the film, which has also now been removed from Twitter - but fans agreed at the time that there wasn't much difference.Bunty: In all my life, I've never heard such a fantastic. Usually in these circumstances, an actress would be given the chance to do a voice test in order to determine the suitability of their pitch and tone, I, however, was not given this opportunity.' Her original statement read: 'The reason they gave is that my voice now sounds "too old" and they want a younger actress to reprise the role. Julia released a statement in July 2020 on Twitter, in a now deleted post, to announce that she wouldn't be involved. Daniel Mays and Romesh Ranganathan, who will be voicing rats Fetcher and Nick, are taking over from Phil Daniels and Timothy Spall in the original.Ĭontroversially, the voice roles of Rocky and Ginger have been replaced, having been originally voiced by Mel Gibson and Julia Sawalha respectively. Which original cast members are returning?Īs reported above, Imelda Staunton will be returning as Bunty, Jane Horrocks as Babs and Lynn Ferguson as Mac.īenjamin Whitrow, the voice of elderly cockerel Fowler, sadly passed away in 2017 - but Harry Potter and Doctor Who star David Bradley will take over his voice. ![]() Taking to Twitter, Netflix announced the date in the caption alongside a short clip from the film, featuring the voices of Zachary Levi and Thandiwe Newton taking over from original voice actors Mel Gibson and Julia Sawalha. Netflix confirmed back in June that Chicken Run: Dawn of the Nugget will be released on the streaming platform on 15th December 2023! Speaking about the announcement, Kristy Matheson, BFI London Film Festival director, said: "We are so excited to be sharing the magic and artistry of the Aardman studio and their favourite feathered friends with audiences this October with a film that’s brimming with fun and has such enormous heart." Along with the world premiere at Southbank Centre’s Royal Festival Hall, there will be preview screenings of the film across the UK. When does the Chicken Run sequel come out?Īhead of its release on Netflix, the British Film Institute have revealed there will be preview screenings in October, as part of the 67th BFI London Film Festival. Chicken Run: Dawn of the Nugget is set for release a whopping 23 years after the original film came out back in 2000, and fans cannot wait to find out when they'll be able to watch the new adventures. You can change this up by using an animal’s name, like “elephant,” or a month like “June.”Ĭhoose from the options shown on the inside flaps. If you happen to pick “orange,” for example, the fortune teller will open and close the cootie catcher based on the number of letters “orange” is made up of. Most cootie catchers place a color’s name on the top flaps. The other participant asks a question then picks whatever is one of the top flaps of the cootie catcher. If you’re the fortune teller, you’ll be the one holding the cootie catcher with both your thumbs and index fingers inside the pockets. You can start writing any “destiny” or “prediction” you want on the folded sides and see if it’s your lucky day! These answers should be applicable to any kind of question, whether that’s about mysteries, a secret admirer, magic, jokes, a trend, other secrets, tricks, and even math! Now you have your own cootie catcher! A simple, cute idea such as this will only take a total time of a few minutes. It should look like a triangle-shaped mouth that can catch cooties - hence the name! ![]() Rotate the paper to the side and pull out all the flaps, making each one look like pockets that can fit your fingers. On the folded sides, you should have inside flaps and outside flaps. Unfold it, then fold it in half again - this time from left to right.įold each corner of the paper towards the inside of the center. Pinch the center together.įold the paper in half from top to bottom. Do this on both sides, so you’ll end up with an X at the center. Start by folding your square sheet in half diagonally. Most origami paper is already square-shaped, but in case you don’t have one, you can just fold and cut off the longer length of a bond-sized paper to end up with a square.
![]() If you pop more enemies, you’ll gain more points and level up faster. The amount you bring in depends on your rank, time you spent flying, and the amount of copters you’ve popped. At the end of each round you’ll gain XP for your copter. Max out your stats to get an edge on your opponents! You can collect upgrades and boosts around the battlefield to increase your speed, your shooting speed, and barrier strength. Keep an eye on your stats bar on the bottom left corner of the screen. If your copter gets caught outside the border when the fog advances, you will only have 10 seconds to escape before you’re done for!īoost your stats. You can see the area the fog will cover on the battlefield marked with a circle. The toxic fog will advance every few seconds. Enemy copters can break down your walls, but if they’re big enough, you may have a chance! ![]() Connect the dots to create barriers around your territory. You can build your own walls with the spacebar as you fly around. You can defend your copter by using the shield upgrades or buy fortifying a strong base. Don’t forget: this is a battle royale style game! Other players will be out to get your copter and knock you out of the rankings. Only one superpower can be used for a limited time - so you’ll have to use them strategically! You can swap your current power for another, so be sure to try them all!ĭefend yourself. Fly over the items from the crate to pick them up and put them to use. Blast the crates by aiming and clicking with your mouse. Items are invaluable to your copter, especially when you’re in a tight spot. As you explore the battlefield, you’ll notice loot boxes and other items scattered around. Swap Superpower: F COPTER ROYALE TIPS & TRICKS ![]() ![]() ![]() The rest of the 56 delegates from 13 colonies actually signed the Declaration of Independence on August 2, 1776, to make it official. August 2nd 1776Īlthough it is widely believed that the Declaration of Independence was signed on July 4th, Only John Hancock and Charles Thompson signed it on July 4. John Adams assisted in the draft of the Declaration of Independence and felt that Independence Day should be celebrated on July 2nd. the vote on Richard Henry Lee’s resolution for independence was nearly unanimous. The Declaration of Independence was signed on August 2nd, 1776.Ĭongress officially declared its independence from Britain on July 2nd, 1776.Independence Day celebrates America’s Independence from Britain.Note: Will Smith stars in five of the top 20 Independence Day Movies 15. Terminator 2 & 3 (Plus Terminator Genysis).Transformers and Transformers Dark of the Moon.Some Notable July 4th Weekend Movie Openings. But Will Smith, he is king of the Independence Day openers with five out of the top 20 hits opening on this date in history. The Biggest Blockbuster movies of the year open on Independence Day weekend. So we thought we’d round them all up in one place for you. There are a lot of other quirky facts about July 4th as well. Hot dogs and fireworks may sound like strange things to mention, but after doing some research on Independence Day, we found out that hot dogs and fireworks have a lot to do with America’s July 4th holiday. Independence Day is a time to break out the barbecue, stock up on fireworks, eat a lot of hot dogs, and drink a lot of Budweiser Beer. Some Notable July 4th Weekend Movie Openings.įacts about Independence Day Celebrations.Philippines celebrates July 4 after Independence from the United States Could have been a Turkey instead of an Eagle Two Presidents Died on the Same Day – July Facts about Independence Day Celebrations. ![]() Now that you have a backup of all vital data, you can go for the Windows 11 upgrade. Step 4: Start the Windows 11 Installation Then, choose an encryption method (AES-256, for example), and you’re done - nobody can access that backup unless they have your password. To do that, go to the “Advanced” tab in the Disk backup options menu and type a password in the “Backup protection” fields below. You can also apply encryption to the hard drive backup. If you've done so, you won’t need to reinstall Windows from scratch - you can simply restore the bootable partition and proceed as if the OS never failed. Here, you can also choose the “ Acronis Survival Kit” feature, which enables a bootable partition to recover your backups even if your operating system fails. You’ll then be prompted to choose what data to backup and set up exceptions. To create a hard drive backup, you can connect the hard drive to your system and select “Add a backup”. If you don’t, the solution will create a new backup and transfer it to the cloud. The software will synchronize your current backup to save time if you have an existing backup in the chosen storage location.(the latter is only possible if you have enough space on the said partition) After you pick “Entire PC”, choose a backup option to copy your system to the Acronis cloud, an external hard drive, or a separate partition on your system drive. ![]() This way, you’ll be able to restore your whole system in case the upgrade fails or even migrate the backup to a different device or a virtual machine. Here, you’d want to pick “Entire PC” to copy the entirety of your computer and put the copy safely in storage. Pick a backup option you can go for Entire PC, Disks and partitions, Files and Folders, and Mobile device backups.Launch Acronis Cyber Protect Home Office.Here’s how to do it with Acronis Cyber Protect Home Office: To avoid any potential data loss, it’s best to backup your data before any major updates. And such scenarios have happened in the past with Windows systems. However annoying that may be, it's better than losing your data altogether. You’d have to install them one by one all over again. We’ve mentioned bricked systems in the last section, which aren’t a pleasure to deal with.Ī bricked system is when an upgrade fails so dramatically that it renders your computer useless (or nearly so).Įven if the computer is salvageable, it's possible to lose all your applications after an upgrade. Jess was available on short notice, efficient, easy to work with, and did a great job. I was grateful that she had the sense of humor and spirit to appreciate Super Problems a lot of editors wouldn't. "My story is 100% sharper and more coherent because of Jess' work. The feedback she gives is top-notch even in copy-editing/proofreading, she works along with the author in ensuring that the novel's plot, world-building, and characters are coherent and written in the best possible way." Editage Book Editing, Translation, and Cover Design Services. "Jess is very meticulous and detailed in the editing process, and she understands the author's style and tries to retain it as much as possible. .uk Literary Consultants and Professional Book Editors. I'll definitely work with her again in the future!" She also gave me the option to spread my payments, which I greatly appreciated since an editor is expensive but she's worth the money. That means Writing Tips Oasis receive a small of the sale if you purchase The Novel Factory, but at no extra cost to you: 1. Edit with confidence, and publish with pride. ![]() We evaluate the foundation of your novel to ensure it is strong. ![]() The only online self-editing platform built from real, best-selling books. Structural Editing is the best service for fiction writers. The following banner is an affiliate one. Meet the only writing and editing platform that helps you crack the code of real-world publishing standards. She replies fast to emails and even kept me up-to-date about the progress while she was editing. If you’ve written a novel and need to get it edited, below we’ve featured 17 of the best freelance fiction editors. "I found Jess great and easy to work with. ![]() Not only can employees access Word and Outlook from any browser or via Android and iOS applications, documents can also be accessed and stored in the cloud whilst on the go through OneDrive (which is offered under all Office 365 plans) or SharePoint Online (which can be bought as part of Office 365 or separately) – increasing productivity in the process. However, when around three-quarters of employees now use personal mobile devices for work purposes, the cloud benefits of Office 365 are endless. Microsoft Office 2016 can only be installed on one computer per licence, and users typically access and store documents via a local drive or shared server. However, when you consider that support will eventually end for this software, and that cloud has led many employees to expect the latest updates as a matter of course, there’s something to be said for Office 365’s rapid and low-maintenance update cycle. Some businesses happily plug along with older versions of Office for years, so this may not be an important differentiator to every firm. However, Office 365 offers continual upgrades to the latest features as part of your subscription, making outdated software a thing of the past. ![]() Purchasing Microsoft Office 2016 will only provide you with the software features that exist at that point in time and you will only receive free security and bug updates. Access to software updates in Office 2016 vs Office 365 Office 365 enables a seamless integration between the two – you can use Office 365 to edit documents created in Office 2016 or earlier, or move documents created by Microsoft Office into the cloud should you wish. Compatibility with old Office documentsĪnother concern for many businesses is how Office 365 integrates with existing documents created in Microsoft Office – either internally or externally (such as by clients). Microsoft Office 2016 may therefore trump the Office 365 Business Essentials package in this regard, but Office 365 Business and Office 365 Business Premium both offer the option of installing Outlook, Powerpoint, Word, Excel and Access to your desktop. Whilst some may be happy to use web browser applications for Microsoft Office, others desire the instinctive element of desktop-installed software. From university projects to places of work, most people will have used Microsoft Office extensively throughout their lives. One of the reasons the Office suite continues to be the go-to software for businesses is that everyone knows how to use it (or at least they should do). Here are the top seven pressing issues to be aware of when choosing between Microsoft Office 2016 and Office 365. Microsoft’s cloud version brings some added benefits – and often additional questions, such as compatibility and usability issues. The differences do not end there, however. Meanwhile, Office 365 is a cloud solution that offers access to the Microsoft suite of applications for a monthly or yearly fee. Microsoft Office 2016 is the latest software upgrade you are accustomed to: a one-off fee grants you a licence to install the software on a computer within your network. However, whereas once you simply paid for the latest update to access new features, you now have two options: upgrade to Microsoft Office 2016 (the 17 th software release) for a one time fee, or consider Office 365 – the monthly subscription service of the software that includes other cloud benefits. Microsoft Office has long been the de-facto business suite for organisations of all sizes. ![]() ![]() You can find the original version here: Office 365 and Office 2016 – what’s the difference? Resources & insights What’s the difference between Office 2016 and Office 365? ĭisclaimer: This is a new version of a blog we originally wrote in August 2015, a few months before Office 2016 hit the shelves, which we’ve now updated for a 2017 audience. ![]() Can be made with fresh or canned salmon.Made in under 30 minutes, perfect for busy weeknights.Serve with a creamy dill sauce for dipping.Flavorful mix of red bell pepper, chives and a lemon dill mixture.Salmon patties are crispy on the outside, tender and flaky inside.❤️ Why You’ll Love this Salmon Patties Recipe What goes good on top of salmon patties?.Is salmon OK for keto? How many carbs are in a salmon patty?.How do you keep salmon patties from falling apart when frying?.What can I use instead of breadcrumbs in salmon patties?.❤️ Why You’ll Love this Salmon Patties Recipe.If you love breakfast for dinner (or brinner) like I do, then you’ll adore this quick breakfast idea. ![]() Here are some toppings you can pile onto these bacon and egg quesadillas: This is a super simple breakfast idea that can be enjoyed on its own, or paired with dipping sauces and sides to round out your morning meal. This will help it achieve that golden brown color.
Of course if you're getting mobbed by Lesser Wisps then feel free to toss a bomb/spear their way, as a mob of Wisps in general is a recipe for a run-end. Since Arti's secondary is generally nuke-level at all times (for the most part), anyone near enough the crossfire will die anyway, so the sooner you melt the boss, the sooner you get the item and move on with the small fry. It's a hard sell, but if I have enough Lensmaker Glasses, I can usually still keep reliably hitting crits for most of my attacks.įor solo runs, prioritize killing the boss first. Forgive Me Please is also nice to have when you have a ton of on-kill proc items already (Topaz Brooch, Monster Tooth, Fresh Meat, Desk Plant, Ceremonial Dagger, etc.), since that thing will just give you those benefits 8 times, potentially killing everything around the doll and maxing your health and temp shields back up top.ĭunno if you'd want to, but when I'm running Arti for long runs, I usually gamble with Purity (the Lunar Item that cuts 2s off all your cooldowns but reduces your luck) when I have enough crits. ![]() Personally, I just bring either the Ocular HUD to help melt enemies faster (especially late game) or Foreign Fruit to have a backup heal when things get too heated and I'm uncertain if the time it takes to proc my Medkit is too long before the next hit lands. When you do get hit, all aggro on you is lost (Stealth Kit), and within a second, you heal back a good portion of your lost health (Medkit) and begin rapidly healing whatever you couldn't heal back while repositioning (CSlug).įor Equipment, people usually say get the Primordial Cube (black hole sucky-suck) to maximize your Bomb/Spear damage. Leech Seed/Scythe will keep you topped off if you're hovering and killing off mobs while you get peppered by some Lesser Wisps, and Stealth Kit, Medkit, plus the Slugs are basically insurance in the event you take a strong hit. I usually go for Ukelele or Will-o-Wisp first since those can hit the most mobs when triggered, but you can build to your preference.įor survivability, I'd recommend having at least a Leech Seed/Harvester's Scythe, a Stealth Kit, Medkit, and Cautious Slugs. When you're reliably landing crits for a good portion of all your attacks, you can start building off your proc items. This ensures that whenever you land a hit, it will always be double of what you can currently deal (with the added bonus of proc-ing and crit-related items you have). ![]() ![]() She's also not very high-DPS (all her skills work off of a cooldown), so I'd recommend just building crits on her (Lensmaker Glasses, Harvester Scythe, Splatterspleen, etc.) to get the ball rolling early on. Usually just the WQ is enough to augment your movement on the ground, but if you're not comfy with pressing jump while running all the time, you can just opt for an Energy Drink, though this runs the risk of running into smaller mobs that may block your path (and maybe two-shot you). TLDR For Artificer, build crits, get wax quail, stealth kit, medkit, and cautious slug.įor Arti, start with supplementing her movement with a Wax Quail (or Energy Drink). |
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